Composite

Part:BBa_K2128006:Design

Designed by: David Sanderson   Group: iGEM16_Genspace   (2016-08-13)


RFP with H. Dujardini ACTN Promoter, RBS and Terminator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 716
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 716
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 716
    Illegal BglII site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 716
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 716
    Illegal AgeI site found at 766
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58
    Illegal SapI site found at 280


Design Notes

We codon optimized the RFP ORF for C. Elegans as no optimization for H. Dujardini or other Tardigrade species is available.


Source

BBa_E1010, H. Dujardini

References